General Cell Based Assays
Eurofins Discoverx PathHunter Human GPR22 β-Arrestin Cell-Based Assay Kit (U2OS)
The PathHunter Human GPR22 β-Arrestin Stable Cell Line Assay (U2OS) contains a stable clonal cell line used to measure GPR22 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human EDNRA β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human EDNRA β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure EDNRA (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human GPR137 β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human GPR137 β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure GPR137 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Enzo Life Sciences FLUOR DE LYS SIRT5 fluorometric drug discovery assay kit (100 tests)
The SIRT5 Fluorometric Drug Discovery Kit is a complete assay system designed to measure the lysyl desuccinylase activity of the recombinant human SIRT5 included in the kit. A black 96-well microplate is packaged with the kit, but it should be noted that reagents of the FLUOR DE LYS system have also been successfully employed in other formats, including cuvettes and 384-well plates. The SIRT5 Fluorometric Activity Assay is based on the unique FLUOR DE LYS-Succinyl Substrate/Developer combination. The FLUOR DE LYS-Succinyl Substrate, which comprises a single lysine residue, Nε-succinylated on its side-chain, is first incubated with human recombinant SIRT5 together with the cosubstrate NAD+. Desuccinylation of FLUOR DE LYS-Succinyl sensitizes it so that, in the second step, treatment with the FLUOR DE LYS Developer produces a fluorophore. Alternative name: Sirtuin 5 fluorescent assay kit. Applications: Fluorescent detection, HTS Cell-based assays, Activity assay.
Revvity Health Sciences Inc AlphaLISA SureFire Ultra Multiplex Human & Mouse Phospho-SMAD2 (Ser465/467 & Total) Detection Kit, 50,000 Assay Points
AlphaLISA SureFire Ultra Multiplex Human & Mouse Phospho-SMAD2 (Ser465/467 & Total) Detection Kit, 50,000 Assay Points
Eurofins Discoverx InCELL Hunter eXpress HEK 293 BRDT(1,2) Bromodomain Assay, 10-plate Kit. (96-0046E1CP7L)
The InCELL Hunter eXpress HEK 293 BRDT(1,2) Bromodomain Assay provides an easy-to-use cell based assay to study small molecule drug candidates that engage with regulators of epigenetics pathways. This kit assesses compound-engagement-based stabilization of BRDT(1,2) via detection of BRDT(1,2) levels. This product uses EFC technology. The cell line included overexpresses BRDT(1,2) target protein fused to ProLabel. In the absence of compound that binds and stabilizes the target, this protein is degraded and no EFC signal is produced. When compound engages and stabilizes the target, EA contained in the detection reagent produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Abcam Cambinol, SIRT1/2 inhibitor, 5MG
MW 360.4 Da, Purity >98%. SIRT1/2 inhibitor (IC₅₀ values are 56 and 59 μM at SIRT1 and SIRT2 respectively). Class III HDAC inhibitor, with no inhibition of class I and II. Promotes G₂ cell cycle arrest. Antitumour activity in vitro and in vivo.
The product is subject to the following: Abcam Restricted Use Statement
Eurofins Discoverx cAMP Hunter eXpress ADRB2 (B2AR) CHO-K1 GPCR Assay, 10-Plate Kit. (95-0121E2CP2L)
The cAMP Hunter ADRB2 (B2AR) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. isoproterenol) based activation of ADRB2 GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type ADRB2, a naturally Gs coupled GPCR. Upon activation of ADRB2, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress GPR141 CHO-K1 β-Arrestin Orphan GPCR Assay, 10-Plate Kit. (93-0385E2ACP1L)
The PathHunter GPR141 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR141 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR141 and EA-tagged β-Arrestin-2. Activation of GPR141 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter eXpress PTHR2 CHO-K1 GPCR Assay, 2-Plate Kit. (95-0057E2CP2M)
The cAMP Hunter PTHR2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. parathyroid hormone, TIP 39) based activation of PTHR2 GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type PTHR2, a naturally Gs coupled GPCR. Upon activation of PTHR2, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery U-PLEX Human IP-10 ASY
The Meso Scale Discovery U-PLEX Human IP-10 Assay is an electrochemiluminescent immunoassay for the quantitative measurement of human interferon gamma-induced protein 10 (IP-10) in serum plasma and cell culture supernatants It utilizes MSD s proprietary SECTOR Imager 6000 for high sensitivity and a wide dynamic range The assay has high specificity with no cross-reactivity and 1% interference It requires 50 L or less sample volume and has an ultra-low lower limit of detection of 45 fg/mL The assay takes 3 5 hours for completion This powerful and sensitive assay allows reliable quantification of IP-10 across a wide range of concentrations
Abcam Amylase Assay Kit (Colorimetric)
Amylase Assay Kit (Colorimetric)
The product is subject to the following: Abcam Restricted Use Statement
Eurofins Discoverx PathHunter eXpress mUTR2 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0525E2MCP0M)
The PathHunter mUTR2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. urotensin II) based activation of mUTR2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mUTR2 and EA-tagged β-Arrestin-2. Activation of mUTR2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress EDG7 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0636E2CP13L)
The PathHunter EDG7 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. LPA) based activation of EDG7 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged EDG7 and EA-tagged β-Arrestin-2. Activation of EDG7 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress mOPRD1 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0611E2MCP0M)
The PathHunter mOPRD1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. DADLE) based activation of mOPRD1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mOPRD1 and EA-tagged β-Arrestin-2. Activation of mOPRD1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.